Development of an industrial line parametric editor in virtual reality via Autodesk Vred

This thesis describes the development of a parametric virtual reality editor for an industrial line for quality inspection, packaging and palletizing of ceramic tiles. This project has been developed entirely within the R&D laboratory of Sacmi S.C., and includes a complete study of the implementation of an application of this kind, from the initial software and hardware selection phase, through the design of algorithms and code programming - in Python language - to the creation of interactive tools and widgets specifically designed for the exploration of the environment, interaction with the scene and automatic production of outputs, with the aim of framing this editor within the production process of automatic lines for the realization of ceramic materials. The editor is defined as "parametric" due to the fact that it requires the user to provide as input a series of values necessary for the configuration of the lines - up to a maximum of three, each with a set of independent parameters - and then it automatically proceeds to compute the geometries and animate the scene, allowing the user to enter in an immersive and realistic context, in order to make a choice among the proposed lines. Communication protocols have also been developed between different scenes for the transfer of data from one to the other, and for the transfer of the user between the different environments through commands given directly in virtual reality. Subsequently, a phase aimed at optimizing the simulation was addressed, in order to increase its stability and decrease the possible stress induced during the presence in VR. Finally, an early study was carried out on multi-user experience – i.e. the presence of several users in the same scene - with the aim of testing possible developments in the field of trade fairs and employee training

Whole Genome Characterization of Orthopoxvirus (OPV) Abatino, a Zoonotic Virus Representing a Putative Novel Clade of Old World Orthopoxviruses

Orthopoxviruses (OPVs) are diffused over the complete Eurasian continent, but previously described strains are mostly from northern Europe, and few infections have been reported from Italy. Here we present the extended genomic characterization of OPV Abatino, a novel OPV isolated in Italy from an infected Tonkean macaque, with zoonotic potential. Phylogenetic analysis based on 102 conserved OPV genes (core gene set) showed that OPV Abatino is most closely related to the Ectromelia virus species (ECTV), although placed on a separate branch of the phylogenetic tree, bringing substantial support to the hypothesis that this strain may be part of a novel OPV clade. Extending the analysis to the entire set of genes (coding sequences, CDS) further substantiated this hypothesis. In fact the genome of OPV Abatino included more CDS than ECTV; most of the extra genes (mainly located in the terminal genome regions), showed the highest similarity with cowpox virus (CPXV); however vaccinia virus (VACV) and monkeypox virus (MPXV) were the closest OPV for certain CDS. These findings suggest that OPV Abatino could be the result of complex evolutionary events, diverging from any other previously described OPV, and may indicate that previously reported cases in Italy could represent the tip of the iceberg yet to be explored.Peer Reviewe

Fatal Outbreak in Tonkean Macaques Caused by Possibly Novel Orthopoxvirus, Italy, January 2015

In January 2015, during a 3-week period, 12 captive Tonkean macacques at a sanctuary in Italy died. An orthopoxvirus infection was suspected because of negative-staining electron microscopy results. The diagnosis was confirmed by histology, virus isolation, and molecular analysis performed on different organs from all animals. An epidemiologic investigation was unable to define the infection source in the surrounding area. Trapped rodents were negative by virologic testing, but specific IgG was detected in 27.27% of small rodents and 14.28% of rats. An attenuated live vaccine was administered to the susceptible monkey population, and no adverse reactions were observed; a detectable humoral immune response was induced in most of the vaccinated animals. We performed molecular characterization of the orthopoxvirus isolate by next-generation sequencing. According to the phylogenetic analysis of the 9 conserved genes, the virus could be part of a novel clade, lying between cowpox and ectromelia viruses